Quiz on Haploid Culture

Haploid plants are saprophytes which have gametophyte chromosomal number.

So, this topic is based on this concept. A score of above 75% will ensure that your knowledge about this topic is good.

More than one option can be true for any given question.

Questions:

1. What is another name for chromosome elimination technique?
   1. Delayed pollination
   2. Bulbosum technique
   3. Distant hybridization
   4. All of the above
2. Which two plants are used for Bulbosum technique?
   1. Datura innoxia and Hordeum vulgare
   2. Hordeum vulgare and Hordeum bulbosum
   3. Hordeum bulbosum and Lycopersicon erythrorizon
   4. Lycopersicon erythrorizon and Hordeum vulgare
3. Which plant chromosome is eliminated in Bulbosum technique?
   1. Hordeum bulbosum
   2. Lycopersicon erythrorizon
   3. Datura innoxia
   4. Hordeum vulgare
4. Which of the following statements is true for Brassica napus?
   1. Its older pollens inhibit young pollen grains development by secreting toxins
   2. Its genotype is very responsive
   3. Its microspore divides to give two equal sized daughter cells, where both cells divide to contribute to the saprophyte
   4. All of the above
5. Which of the following statements is true for Datura innoxia?
   1. In this plant, anther culture technique was first successfully developed by Guha and Maheshwari
   2. It shows good response when culture media contains 2-4% sucrose
   3. The best stage for pollen culture is just before or after first mitosis of pollen
   4. No embryo is formed if temperature is below 20°C for anther culture
   5. Irradiation and centrifugation is effective for its culture
   6. Its microspore divides to give large vegetative cell and small generative cell, where both cells divide to contribute to the saprophyte
   7. All of the above
6. Which of the following statements is true for Nicotiana tabacum (tobacco)?
   1. To isolate its pollen by density gradient centrifugation, a solution of percoll (55%) and sucrose (4%) is used
   2. It shows good response when culture media contains 2-4% sucrose
   3. Optimum temperature is 25°C. However, a cold shock to anthers at 3-5°C for 2 days or 7-8°C for 12 days increase androgenesis.
   4. Its isolated pollen culture shows good response if grown upon the anther culture of Nicotiana glutonica and Petunia hybrida or petal callus of latter.
   5. Its microspore divides to give large vegetative cell and small generative cell, where vegetative cell divide to contribute to the saprophyte
   6. Irradiation is effective for its culture
   7. All of the above
7. Which of the following statements is true for cereals?
   1. They require N6 or potato media
   2. The best stage for culture appears to be the early or mid uninucleate stage
   3. A cold shock of 4-10°C for 3-28 days is preferred
   4. It shows good response when culture media contains 2-4% sucrose
   5. Optimum temperature is 25°C. However, a cold shock to anthers at 3-5°C for 2 days or 7-8°C for 12 days increase androgenesis.
   6. Irradiation is effective for its culture
   7. All of the above
8. Which of the following statements is true for Solanaceae?
   1. They don't require a growth regulator. But, low level of auxin, cytokinin or gibberellic acid (GA3) is beneficial.
   2. The best stage for culture appears to be the early or mid uninucleate stage
   3. A cold shock of 4-10°C for 3-28 days is preferred
   4. It shows good response when culture media contains 2-4% sucrose
   5. Optimum temperature is 25°C. However, a cold shock to anthers at 3-5°C for 2 days or 7-8°C for 12 days increase androgenesis.
   6. Irradiation is effective for its culture
   7. All of the above
9. Which of the following statements is true for Hyoscyamus niger?
   1. 2, 4-dichlorophenoxyacetic acid (2, 4-D) at concentration of 2mg/ml is beneficial
   2. Its microspore divides to give small vegetative cell and large generative cell, where generative cell divides to contribute to the saprophyte
   3. They don't require a growth regulator. But, low level of auxin, cytokinin or gibberellic acid (GA3) is beneficial.
   4. The best stage for culture appears to be the early or mid uninucleate stage
   5. A cold shock of 4-10°C for 3-28 days is preferred
   6. It shows good response when culture media contains 2-4% sucrose
   7. All of the above
10. Which of the following statements is true for wheat?
    1. 2, 4-D induces callus formation
    2. Coconut milk induces embryogenesis
    3. A cold shock of 4-10°C for 3-28 days is preferred
    4. Feridazone-potassium (gametocidal compound) treatment is beneficial
    5. Its microspore divides to give small vegetative cell and large generative cell, where generative cell divides to contribute to the saprophyte
    6. They don't require a growth regulator. But, low level of auxin, cytokinin or gibberellic acid (GA3) is beneficial.
    7. All of the above
11. Which of the following statements is true for Lolium multiflorum?
    1. They don't require a growth regulator. But, low level of auxin, cytokinin or gibberellic acid (GA3) is beneficial.
    2. Response gets doubled when carbon dioxide concentration is 2%
    3. Ten times decrease in ammonium nitrate concentration enhanced the growth
    4. 2, 4-D induces callus formation
    5. Coconut milk induces embryogenesis
    6. A cold shock to anthers at 3-5°C for 2 days or 7-8°C for 12 days increase androgenesis
    7. All of the above
12. Early binucleate stage is suitable in which plants?
    1. Datura innoxia
    2. Hordeum vulgare
    3. Atropa belladena
    4. Nicotiana sylvestris
13. PMCs (piocarpogonial mother cell) in meiosis I is the optimum stage for which plants?
    1. Tomato
    2. Arabidopsis
    3. Datura innoxia
    4. Hordeum vulgare
14. Which of the following statements is true for rice?
    1. A cold shock of 4-10°C for 3-28 days is preferred
    2. Oryzae sativa inflorescence treated with ethereal for 48 hrs at 10°C is beneficial.
    3. Genotype of japonica rice is more responsive as compared to Indian rice
    4. 2, 4-D induces callus formation
    5. Coconut milk induces embryogenesis
    6. A cold shock to anthers at 3-5°C for 2 days or 7-8°C for 12 days increase androgenesis
    7. All of the above
15. What temperature shock is effective for Brassica campestris?
    1. Cold shock of 3°C for 24 hrs
    2. Cold shock of 10°C for 24 hrs
    3. Heat shock of 35°C for 24 hrs
    4. Heat shock of 45°C for 24 hrs
16. Which of the following effect will reduce androgenesis for Brassica oleraceae?
    1. Cold shock of 10°C for 24 hrs
    2. Heat shock of 35°C for 24 hrs
    3. Presence of small part of filament during anther culture
    4. All of the above
17. Which of the following is effective for barley?
    1. Cold shock of 10°C for 24 hrs
    2. Heat shock of 35°C for 24 hrs
    3. Presence of small part of filament during anther culture
    4. A cold shock of 4-10°C for 3-28 days is preferred
    5. Presence of gelling agents like wheat starch
    6. 2, 4-D induces callus formation
    7. All of the above
18. Who first reported ovary culture in barley in 1976?
    1. Guha and Maheshwari
    2. Guha
    3. Maheshwari
    4. San noem
19. Which of the following is generally required for ovary culture?
    1. Mature embryo sac
    2. Cold treatment
    3. Growth factors
    4. Light
20. What is the efficiency of ovary culture?
    1. 15-20%
    2. 9-10%
    3. 10-15%
    4. 0.2-6%

Answers:

1. b, c
2. b
3. a
4. d
5. g
6. g
7. a, b, c
8. a
9. a, b
10. a, b, c, d
11. b, c
12. c, d
13. a, b
14. a, b, c
15. c
16. c
17. d, e
18. d
19. a, b, c, d
20. d