1. What is the order of reaction, if rate of reaction depends upon the concentration?

1. zero order
2. no order
3. more than zero order
4. first order

2. What does "k" denotes in the equation:

V= k[S]

And what is the unit of "k"?

1. Equilibrium constant and its unit is second2
2. Equilibrium constant and its unit is second-1
3. First order and its unit is second2
4. First order and its unit is second-1

3. What is the order of reaction, if it depends on two different molecules?

1. zero order
2. first order
3. second order
4. third order

4. What is the order of reaction, if it depends on two molecules of same substrate?

1. zero order
2. first order
3. second order
4. third order

5. What is the value of rate constant in second order reaction?

1. Ms
2. M
3. s
4. M-1 s-1

6. What is the relationship between constant and activation energy?

1. no relationship
2. directly proportional
3. inversely proportional
4. can't be determined

7. How enzymes decrease activation energy?

1. restricting the movement of substrate
2. reducing entropy
3. making weak interactions
4. all of the above

8. What is binding energy, DGB?

1. Energy derived from enzyme-substrate interaction
2. Energy derived from enzyme-product interaction
3. Energy derived from product-substrate interaction
4. None of the above

9. Which energy is mainly responsible for lowering activation energy?

1. activation energy
2. transition energy
3. binding energy
4. none of the above

10. In which state weak interactions are optimized?

1. initial state
2. final state
3. intermediate state
4. transition state

11. In which state, substrate specifically to the enzyme active site?

1. initial state
2. final state
3. intermediate state
4. transition state

12. Which scientist proposed lock and key model in 1894?

1. Daniel Koshland
2. Emil Fischer
3. Einstein
4. Michael

13. Who were the first scientists to propose that enzyme must me complementary to the reaction transition state?

1. Michael Polanyi and Haldane
2. Linus Pauling and Haldane
3. Haldane
4. Emil Fischer

14. Who elaborated the work of Michael Polanyi and Haldane in year1946?

1. Daniel Koshland
2. Emil Fischer
3. Linus Pauling
4. Einstein

15. What are the physical and thermodynamic factors which are responsible for lowering of activation energy?

1. reduction in entropy
2. increase in entropy
3. lowers binding energy
4. none of these

16. What is induced fit?

1. when enzyme change shape due to absence of substrate
2. when enzyme do not change shape due to absence of substrate
3. when enzyme change shape due to presence of substrate
4. when enzyme do not change shape due to presence of substrate

17. Who postulated induced fit in year1958?

1. Daniel Koshland
2. Emil Fischer
3. Linus Pauling
4. Einstein

18. Name specific catalytic group which contribute to catalysis?

1. alumina
2. glucose
3. acetic acid
4. none of the above

19. Specific acid-base catalysis uses which ions?

1. H+
2. OH-
3. Both a and b
4. None of the above

20. What is general acid-base catalysis?

1. use proton donors
2. use of proton acceptors
3. no use water as proton acceptor or donor
4. all of the above

ANSWERS:

1. a
2. b
3. c
4. c
5. d
6. b
7. d
8. a
9. c
10. d
11. d
12. b
13. a
14. c
15. a
16. c
17. a
18. d
19. c
20. d

More enzyme quiz related articles, refer:

1. General Biochemistry
2. Quiz on Enzymes
3. Quiz on Enzymes 2
4. Quiz on Enzymes Three
5. Quiz on Enzyme Kinetics

A simple quiz is presented to test your enzyme kinetics knowledge.

Answer the questions with respect to the graph given in that question.

Please note: More than one question might be correct in the questions so answer them accordingly.

a)  In a fermenter, scientist observed some kind of inhibition of its enzyme used to convert its substrate to a product. He observed a graph as shown below when he compared the results with the standard results. Please answer the questions given below to solve his mystery.

1. Graph denotes which kind of plot?
   1. Michaelis-Menten plot
   2. Double reciprocal plot
   3. X-Y plot
   4. None of these
2. Inhibitor is causing which type of inhibition?
   1. Reversible
   2. Competitive
   3. Non- competitive
   4. Mixed
3. What is the effect of inhibitor on Vmax of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient
4. What is the effect of inhibitor on the Km of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient
5. How can he solve the problem?

a) Decrease substrate concentration

b) Maintain the substrate concentration

c) Increase substrate concentration

d) Data insufficient

b) While carrying out a test in his lab, a scientist observed a following graph when he compared with his standard graphs. He could decipher that there is some kind of inhibition to its enzyme urease but could not determine the inhibition type. Please look at the graph and answer the questions given below to solve his mystery.

1. Inhibitor is causing which type of inhibition?
   1. Uncompetitive
   2. Competitive
   3. Non- competitive
   4. Mixed
2. What is the effect of inhibitor on Vmax of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient
3. What is the effect of inhibitor on the Km of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient

c)During the bioassay of enzyme urokinase test sample, some inhibition is observed. Please look at the graph and answer the questions given below to solve the mystery.

1. Inhibitor is of which type?
   1. Uncompetitive
   2. Competitive
   3. Non- competitive
   4. Mixed
2. What is the effect of inhibitor on Vmax of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient
3. What is the effect of inhibitor on the Km of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient

d)During the bioassay of enzyme oxidase test sample, some inhibition is observed. Please look at the graph and answer the questions given below to solve the mystery.

1. Inhibitor is of which type?
   1. Uncompetitive
   2. Competitive
   3. Non- competitive
   4. Mixed
2. What is the effect of inhibitor on Vmax of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient
3. What is the effect of inhibitor on the Km of the reaction?
   1. Same
   2. Decrease
   3. Increase
   4. Data insufficient

Answers:

a)

         i) B

        ii) A, B

        iii) A

        iv) C

       v) C

b)

      i) D

     ii) B

    iii) C

c)   

     i) C

     ii) B

    iii) A

d)

      i) A

     ii) B

    iii) B

For more quizzes on enzyme, please check these links. They are more user friendly and you won't be disappointed:

1. General Biochemistry
2. Quiz on Enzymes
3. Quiz on Enzymes 2
4. Quiz on Enzymes Three

1) Which of the following is a biomolecule?

1. Glucose
2. Hydrochloric acid
3. Iron
4. Sodium chloride

2) Which is the most important biomolecule for the repair of body?

1. Carbohydrates
2. Lipids
3. Proteins
4. Minerals

3) Which metal is used as a cofactor for urease?

1. Zinc
2. Nickel
3. Iron
4. Calcium

4) What is the other name for biocatalyst?

1. Protein
2. Catalyst
3. Enzymes
4. Precursor

5) What are the components of carbohydrates?

1. Carbon
2. Hydrogen
3. Oxygen
4. All of the above

6) Which biomolecule makes our genetic code?

1. Proteins
2. Lipids
3. Carbohydrates
4. Nucleic acids

7) Which biomolecules make up the cell membrane?

1. Carbohydrates
2. Proteins
3. Lipids
4. All of the above

Please note: More than one question might be correct in the above questions so answer them accordingly.

Answers

1. A
2. C
3. B
4. C
5. D
6. D
7. D

More enzyme quiz related articles, refer:

1)      Quiz on Enzymes

2)      Quiz on Enzymes 2

3)      Quiz on Enzymes 3

4)      Quiz on Enzymes 4

5)      Quiz on Enzyme Kinetics

1. What is covalent catalysis?
   • Formation of hydrogen bond between enzyme and substrate
   • Formation of covalent bond between enzyme and substrate
   • Formation of transient covalent bond between enzyme and substrate
   • None of the above
2. How metal ions participate in catalysis?
   • By causing reduction and oxidation reactions between enzyme and substrate
   • By causing ionic interactions between enzyme and substrate
   • All of the above
   • None of the above
3. What is the common enhancing rate of enzymes?
   • 105 to 1017
   • 1012 to 1020
   • 10-17 to 10-9
   • None of the above
4. Enzyme chymotrypsin carries out which type of catalysis?
   • Covalent catalysis
   • General acid base catalysis
   • None of the above
   • Both a and b
5. What is enzyme kinetics?
   • Studying the mechanism of rate of reaction of enzyme
   • Factors affecting enzyme activity
   • Both a and b
   • None of the above
6. What is Vmax?
   • Maximum rate of reaction
   • Rate of reaction increase with increase in enzyme concentration
   • Both a and b
   • None of the above
7. In which year, Victor Henri proposed that the combination of an enzyme with its substrate molecule to form an ES complex is necessary?
   • 1904
   • 1903
   • 1902
   • 1901
8. Who gave the general theory of enzyme action in 1913?
   • Leonor Michaelis and Maud Menten
   • Alfred Michaelis
   • Maud Michaelis
   • Leonor Menten and Maud Michaelis
9. In an enzyme catalyzed reaction, enzyme exists in which form?
   • Free form
   • Combined form
   • ES form
   • All of the above
10. The initial period when ES concentration builds up in an enzyme catalyzed reaction is called?
    • Steady state
    • Initial period
    • Pre steady state
    • Ephemeral state
11. The state which occurs after pre-steady state is called?
    • Pro-steady state
    • Late pre steady state
    • Post steady state
    • Steady state
12. Who introduced the concept of steady state kinetics in 1925?
    • G.E. Briggs and Haldane
    • Michaelis and Menten
    • Briggs
    • Menten
13. What is Km in Michaelis-Menten equation?
    • Michaelis-Menten constant
    • Substrate
    • Enzyme
    • All of the above
14. The initial reaction rate reflects a steady state in which [ES] is constant. This is called?
    • Michaelis and Menten state
    • Steady state assumption
    • Initial state assumption
    • None of the above
15. Which enzymes are said to follow Michaelis-Menten kinetics?
    • Enzymes which show parabolic dependence of rate of reaction and substrate
    • Enzymes which show circular dependence of rate of reaction and substrate
    • Enzymes which show hyperbolic dependence of rate of reaction and substrate
    • None of the above
16. Double-reciprocal plot is also called?
    • Michaelis plot
    • Line plot
    • Lineweaver-Burk plot
    • Inverse plot
17. What is Kd?
    • Dissociation constant
    • Michaelis constant
    • Lineweaver constant
    • None of the above
18. What is the unit of Kcat?
    • Reciprocal of concentration
    • Reciprocal of time
    • Both a and b
    • None of the above
19. Km and Kcat of an enzyme reflects?
    • Cellular environment
    • Concentration of substrate normally encountered in vivo
    • Reaction chemistry
    • None of the above
20. What is the Km of substrate whose concentration in cell is high?
    • Low
    • High
    • Depends on reaction
    • Depends on parameters

ANSWERS:

1. C
2. C
3. A
4. C
5. C
6. A
7. B
8. A
9. D
10. C
11. D
12. A
13. A
14. B
15. C
16. C
17. A
18. B
19. A, B, C
20. B

More enzyme quiz related articles, refer:

1)      General Biochemistry

2)      Quiz on Enzymes

3)      Quiz on Enzymes 2

4)      Quiz on Enzymes 4

5)      Quiz on Enzyme Kinetics